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In this study, a multifunctional liposome was developed for improved anticancer therapy. Briefly, fresh samples were diluted in Millipore water and mixed well. DiR iodide emits weak fluorescence in water but strong fluorescence in cell membrane or lipophilic biomacromolecules. Doc increased M phage arrest by destroying microtubules that were essential for cell division. The issue of drug leakage from the liposomes was improved compared to previous studies. Liposome-cell interactions in vitro: Quantitation of metal content in the silver-assisted growth of gold nanorods. Doc and GNRs were loaded into the liposomes as chemotherapy and thermotherapy agents.